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The Best Proteolytic Enzymes With Lipase, Amylase, Bromelain

Best Proteolytic Enzymes With Lipase, Amylase, Bromelain At A Glance

Organixx P3E Full Spectrum Digestive Enzyme Blend

Our Recommendation

Organixx Enzyme 17 Essential Digestive Enzymes

Organixx’s enzyme blend is extremely cutting edge and features exclusive tri-phase proteolytic protease enzymes, a digestive enzyme blend, and AstraZyme.

Vitauthority Digestion Support Bundle

Best Flavored Digestive Enzyme Powder & Collagen Bundle

Vitauthority Digestion Support Bundle

Vitauthority’s digestion support bundle includes collagen powder and digestive support powder, each available in 5 flavors and 2 flavors, respectively. This is a great option for people who aren’t fans of capsules and want to enjoy taking their digestive enzyme supplement.

Thorne M.F. Bromelain

Best Bromelain Supplement

Thorne M.F. Bromelain

Each serving of 2 capsules provides 1 g of bromelain. This product is intended to promote healing as well as reduce minor swelling and bruising.

Proteolytic enzymes naturally break down proteins into amino acids as part of the digestion process. These amino acids enable healthy body functioning, including combating infection, anti-inflammation, or simply improved digestive functioning.

This article will explore several of these enzymes in detail and provide our recommendations on how you can supplement them to achieve your intended health outcome. Proteolytic enzyme supplementation can occur in many forms including capsule, powder and topical applications – this article will focus on oral supplementation.

Peptidase Function: What Does Peptidase Break Down And What Are Its Benefits?

Proteolytic enzymes, also called proteases, proteinases, or peptidases, are a group of enzymes that naturally (and irreversibly) break long-chained proteins via hydrolysis into shorter fragments, which then form digestible amino acids. This process illustrates essential protein digestion whereby vital amino acids are extracted from your food for normal bodily function. 

These proteases are currently divided into 2 groups: endopeptidases and exopeptidases, depending on their mechanism of action. Endopeptidases cleave sites within long polypeptides, such as pepsin. Exopeptidases cleave either end of the protein molecule, including carboxypeptidases.

The most commonly known proteolytic enzymes reside in different parts of the gastrointestinal system and require different ideal pH conditions. For example, pepsin (found in the stomach) works optimally at pH 1.5-2.5 whereas trypsin (found in the small intestine) is most effective at pH 7.5-8.5. If the pH is not optimal, these enzymes become inactive, and may eventually break down or denature.

Protein breakdown is also called denaturation. Raw food dieters argue that protein denaturation affects food’s nutritional value, but this is not correct. Protein denaturation is a necessary part of the digestion process. Cooking food simply gives your body a head start on the digestive process.

Proteolytic enzymes are largely sold as supplements to reduce inflammation, promote healthy digestion, and combat infection. They are also used in food preparation as meat tenderizers, or medically to break down dead tissue.


Pepsin is the principal enzyme involved in protein digestion in the stomach. It is formed from pepsinogen, which is produced by chief cells in the stomach lining and cleaves itself to form pepsin at low pH. Pepsin specifically breaks down proteins from meat, eggs, dairy products, nuts and seeds.

Despite pepsin’s role in the stomach, it is not considered an essential enzyme and causes issues associated with laryngopharyngeal reflux or acid reflux

However, pepsin supplementation does offer health benefits in particular cohorts. Patients with conditions which predispose them to pancreatic dysfunction (i.e. cystic fibrosis) are supplemented with pepsin to improve GI functioning. Commercial pepsin is derived from pig, cow or sheep gastric mucosa, and often supplemented with Betaine HCl to aid digestion. 

Pepsin supplements may also aid in fighting bacterial infections in the stomach. In vitro studies have demonstrated that pepsin supplementation is effective in reducing bacterial cultures such as Escherichia coli and Helicobacter pylori

Betaine HCl

pH is an important mediator of essential enzyme activity in digestion. Betaine HCl is a synthetic substance which restores ideal gastric acidity in people suffering from hypochlorhydria (low stomach acid production). This enables digestive enzymes, like pepsin, to function properly.

In patients who produce insufficient stomach acid, betaine HCl aids absorption of orally administered weakly basic drugs that are pH-dependent soluble. Further, betaine HCl can mitigate potential drug-drug interactions between these drugs.

Dietary supplementation of betaine HCl with pepsin can also relieve acid reflux and IBS-associated symptoms including bloating, sleep disturbance and lower abdominal pain. This is due to betaine HCl and pepsin stabilising physiological processes within the gut, and improving protein denaturation and nutritional absorbance.

The standard practice for supplementing with Betaine HCl involves starting with 1 tablet per meal and slowly raising that dose until you feel a warming sensation in your stomach. Once you feel this, lower the dose by one tablet and this is your standard dosing level.  In other words, you want to use the maximum dose that does not produce that warming sensation.


Bromelain, also known as bromelin, is a protein-digesting enzyme derived from all living parts of the pineapple plant. Historically, Bromelain was a common solution for treating inflammation and indigestion in Central and South America. This is possibly due to proteolytic components contained within bromelain, including proteinases.

Today, bromelain is researched intensively for its potential to treat cancer, inflammation and infection. For example, mice cancerous colorectal cell line growth in vitro is inhibited under bromelain application.

Concerning inflammation, bromelain may treat rheumatoid arthritis, osteoarthritis and muscle soreness. A recent review has even suggested that bromelain reduces the activation of inflammatory factors involved in IBS.

Finally, bromelain has antimicrobial effects. For example, in piglet small intestine, bromelain prevents bacteria in the gut from attaching to intestinal mucosa cells. In addition, bromelain offers indirect antimicrobial activity through necrotic tissue debridement – debridement means cleaning wounds in the skin by clearing away dead tissue. This method is possibly more effective than surgical debridement, though it is only achieved through topical application.

To attain bromelain’s benefits, bromelain may be taken alone or in conjunction with other medications – bromelain has been shown to improve antibiotic absorption.

For wound debridement, topical application is necessary to remove necrotic tissue from burn areas, and for areas of swelling (such as osteoarthritis), oral application is advised.

A study has shown that bromelain has blood thinning properties and boosts warfarin’s effect harmfully in bovine and rodent models. Other blood thinners whose activity may be increased by bromelain include aspirin, heparin and clopidogrel. Consult your doctor if you are taking blood thinners before supplementing with bromelain.


Papain, also known as papaya proteinase 1 (cysteine protease enzyme), is a proteolytic enzyme extracted from the latex of papaya and mountain papaya. Latex is collected after scoring the neck of the fruit, where it may either dry on the fruit or drip into a container. Thus, people with latex or papaya allergies should avoid papain.

Papain is mainly used to digest tough meat fibres. This stems from its original use in South America to tenderize meat. In pure form it is highly potent; 8 drops of papain tenderizes 1 kg of meat. You will typically find papain in powder form for meat tenderizing. 

Like bromelain, papain is effective in debridement therapies. For example, Accuzyme Papain-Urea Debriding Ointment. Further, researchers have observed enhanced skin absorption under papain treatment to remove scar visibility in rodent models. Before investing in this treatment, please visit your doctor – the FDA is currently aware that certain topical papain formulations can cause severe allergic reactions.

Research into other possible papain benefits remains slim despite papain’s traditional use. Papain may aid in treating IBS-associated constipation due to observed increased cysteine levels in constipated patients, though this remains speculatory. Similarly, papain was thought to be anti-inflammatory, though research is lacking. More promisingly, papain taken with bromelain and fungal proteases may aid in muscle recovery after training.

The Best Proteolytic Enzyme Supplements – Buy Proteolytic Enzymes From These Trusted Brands

Organixx P3E Full Spectrum Digestive Enzyme Blend

Our Recommendation – Best Amylase, Bromelain Lipase

Organixx Enzyme 17 Essential Digestive Enzymes

Organixx’s Enzyme 17 contains a wide variety of proteases, including bromelain. Each protease works optimally at a different pH level in the gastrointestinal system, promoting healthy digestion. For those of you who are lactose-intolerant, this product also contains lactase, which aids in dairy consumption. Fat-burning lipases are also worth noting as they can help break down fats if you’re transitioning to/on a high-fat diet. 

Unlike other products, astrazyme is also present – a combination of Panax ginseng, astragalus and proteolytic enzymes with enhanced protein digestion. 3 capsules are recommended as the daily serving size. On the whole, Organixx’s enzyme blend is extremely cutting edge.

Proteolytic enzymes, or proteases, are often sold as combination supplements to ensure that different proteins are broken down, maximising its effect in the body – each proteolytic enzyme has a unique active site which only acts on a specific protein. One example of a common protease supplement is a combination of amylase, bromelain, lipase.

Therefore, prior to consuming combined enzyme supplements, it is important to check what enzymes are contained within each product in case you are allergic, for example, to papaya. This is especially important to check in meat tenderizers, such as papain tenderizers.

Remember to avoid bromelain if you are taking any anticoagulants, such as warfarin and aspirin, due to bromelain’s blood thinning effect.

All protease supplements require systemic absorption which means you should take these supplements 15-30 minutes before meals on an empty stomach.

Thorne M.F. Bromelain

Best Bromelain supplement

Thorne M.F. Bromelain

Thorne’s M.F. Bromelain is the best bromelain product on the market. Every bottle has 60 capsules. Each 2 capsule serving contains a high, safe dose of pure bromelain to tend to your needs. Please note that if you are on anticoagulant therapy, this product is not safe for you. As bromelain is derived from the pineapple plant, this product is suitable for vegetarians. Overall, Thorne Research guarantees a very high-quality manufactured product.

Thorne Betaine HCL With Pepsin

Betaine HCL with Pepsin

Thorne Betaine HCL With Pepsin

Thorne’s Betaine HCl & Pepsin product is ideal for people who suffer from hypochlorhydria, IBS or are on a high protein diet. Each bottle contains 225 capsules. Remember to find your perfect dosing amount by slowly increasing from 1 tablet a day until you feel that warm sensation in your stomach. Decrease the dose slightly and continue at that dosage. Please note that pepsin is derived from pigs and is not vegetarian-friendly. The positive reviews concerning this product demonstrate its efficacy in treating indigestion.

Vitauthority Digestion Support Bundle

Contains lipase, amylase, bromelain

Vitauthority Digestion Support Bundle

Vitauthority’s digestion support bundle includes two products: collagen powder and digestive support powder – each available in 5 flavours and 2 flavours, respectively. The collagen powder contains collagen to promote healthy skin, bones and joints, and hyaluronic acid which increases collagen production in the body. Collagen is derived from animal products so this product is not suitable for vegetarians. 
In the digestive support supplement are proteases, amylases, bromelain, lipases (fat-digesting), and more. Due to the presence of bromelain, please avoid this product if you are on blood thinners. Apple cider vinegar is also in this product and reduces harmful blood lipids. Mix 1 scoop of each with your favourite beverage or recipe. Vitauthority offers this product as a one time purchase or you can subscribe and save – a great purchase!

Runner Up: Best Bromelain Supplement

Transformation Enzymes Protease Blend

Transformation Enzymes Protease Blend contains vital proteolytic enzymes including peptidases, bromelain and papain. Please note that if you are allergic to latex or papaya, this product is not safe for consumption due to the inclusion of papain. 

Each bottle contains 120 capsules. You should take 1 capsule twice daily on an empty stomach to achieve its full effect. Exceptionally positive reviews have named this product as the best on the market, with the only complaint being it is frequently out of stock.  There is a subscription option available for people who plan to use it regularly and would like to save some money on it.

Editor’s note: we are regularly updating this review. If you see any problems, weird interpretations of the data, or just want to say hi, please reach out to

About the author

Lucy is a UK-based freelance writer focusing on biological content, whether it may involve animal biology or health and well being. Having achieved a First Class Zoology degree at the University of Bristol, Lucy has a diverse knowledge base and enjoys writing for others. Lucy is also a medical student in London who enjoys, in her free time, weight lifting at the gym or hiking along precarious routes in the great outdoors.

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